Coding

Part:BBa_K4488000:Design

Designed by: Jessica Zhang   Group: iGEM22_Sydney_Australia   (2022-09-30)


Neae: N terminus of 𝛃-intimin for Surface Display in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed banned restriction sites.


Source

Adapted from Salema & Fernandez (2017).

References

Salema, V., & Fernández, L. Á. (2017). Escherichia coli surface display for the selection of nanobodies. Microbial Biotechnology, 10(6), 1468-1484. https://doi.org/https://doi.org/10.1111/1751-7915.12819